Protein structural dynamics and folding using HDX-MS

26 February 2020

Get in touch!

Interested?

For more information please contact Prof. dr. Tassos Economou, tel.: +32 16 37 92 73, mail: tassos.economou@kuleuven.be or Mrs. Lily Karamanou, tel.: +32 16 37 92 08, mail: lily.karamanou@kuleuven.be.

You can apply for this job no later than May 31, 2020 via the online application tool

KU Leuven seeks to foster an environment where all talents can flourish, regardless of gender, age, cultural background, nationality or impairments. If you have any questions relating to accessibility or support, please contact us at diversiteit.HR@kuleuven.be.

Apply before 31 May 2020

The Laboratory of Molecular Bacteriology, Rega Institute, Department of Microbiology, Immunology and Transplantation, KU Leuven (https://rega.kuleuven.be/bac/economou) wishes to recruit 2 postdoctoral fellows in the following projects:

a. Secretory protein non-folding and folding and how it is regulated by intrinsic and extrinsic (e.g. chaperones) factors.

b. Structural dynamics of membrane associated/embedded channels and motors.

The lab studies protein trafficking through the Sec and the Type III secretion systems (Tsirigotaki 2018 Structure; Chatzi 2017 JCellBio; Portaliou 2018 EMBO J; Saio
 2014 Science 344, 6184; Gouridis 2013 Molecular Cell 52, 655; Chen 2011 Molecular Cell 44, 734; Gouridis 2009 Nature 462, 363; Gelis 2007
Cell 131, 756) and disordered/flexible human proteins involved in disease and chaperoning (e.g. Bcl-2 and IP3-regulate Ca2++ channel, prolyl oligopeptidase, -secretase; Monaco 2017 FEBS; Tsirigotaki 2017 SciReports). We have a very international lab environment and use multi-disciplinary approaches that combine molecular microbiology and genetics, recombinant DNA technologies, enzymology, protein chemistry, protein biophysics (including Hydrogen Deuterium Exchange/Native/nanoLC mass spectrometries, Isothermal Titration Calorimetry, Circular Dichroism, ensemble fluorescence, size exclusion chromatography coupled online to static and dynamic laser light scattering detection) structural biology, single molecule FRET. All methods are applicable on lab equipment.

Our lab MS, a Waters Synapt G2, is equipped with a nanoAcquity UPLC and HDX capabilities. We also have direct access to two Orbitraps: a Q-exactive and an Elite. We use MassLynx, PLGS, DynamiX and in-house developed software.

Website unit

Responsibilities

Planning and execution of experimentsWriting of papersPresentation at meetings

Profile

· hold a PhD in Chemistry or Biochemistry or Physics or a related degree

· have experience in using HDX-MS or other MS methods.

· be experienced in protein purification and biochemistry.

· have a genuine interest in research and scientific discovery and particularly in protein structural dynamics, protein interactions and folding.

· Additional experience in biophysical methods and/or bioinformatics will be appreciated.

· have a good command of English

Offer

Full-time postdoctoral position